CD9 Marks Flt3+ Multipotent Hematopoietic Progenitors within Lsk Cells

FLT3 expression initiates in fully multipotent mouse hematopoietic progenitor cells.

Lymphoid-primed multipotent progenitors with down-regulated megakaryocyte-erythroid (MkE) potential are restricted to cells with high levels of cell-surface FLT3 expression, whereas HSCs and MkE progenitors lack detectable cell-surface FLT3. These findings are compatible with FLT3 cell-surface expression not being detectable in the fully multipotent stem/progenitor cell compartment in mice. If ...

Matters Arising New Evidence Supporting Megakaryocyte- Erythrocyte Potential of Flk2/Flt3 Multipotent Hematopoietic Progenitors

E. Camilla Forsberg,* Thomas Serwold, Scott Kogan, Irving L. Weissman, and Emmanuelle Passegué* 1 Institute of Stem Cell Biology and Regenerative Medicine, Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA Department of Laboratory Medicine and Comprehensive Cancer Center, University of California, San Francisco, CA 94143, USA The...

New Evidence Supporting Megakaryocyte-Erythrocyte Potential of Flk2/Flt3+ Multipotent Hematopoietic Progenitors

A model of hematopoietic development wherein multipotentiality is conserved until segregation of myeloid and lymphoid potential has recently been challenged, proposing that megakaryocyte/erythrocyte (MegE) potential is lost in Flk2/Flt3-expressing early progenitors. Here, we used sensitive in vivo approaches to quantitatively and kinetically assess the MegE potential of hematopoietic stem cells...

Progenitors in Mouse Bone Marrow Heterogeneity of Flt3-Expressing Multipotent

Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors

PU.1 is an Ets family transcription factor that is essential for fetal liver hematopoiesis. We have generated a PU.1(gfp) reporter strain that allowed us to examine the expression of PU.1 in all hematopoietic cell lineages and their early progenitors. Within the bone marrow progenitor compartment, PU.1 is highly expressed in the hematopoietic stem cell, the common lymphoid progenitor, and a pro...